Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Trends Biotechnol. 2008 Jun;26(6):285-7. doi: 10.1016/j.tibtech.2008.03.005. Epub 2008 Apr 24.

Engineered polymerases amplify the potential of ancient DNA.

Author information

  • Department of Biology, 326 Mueller Laboratory, The Pennsylvania State University, University Park, PA 16801, USA. beth.shapiro@psu.edu

Abstract

The generation of genomic data from mammoths and Neanderthals has reinvigorated discussion about whether extinct species could be brought back within the foreseeable future. However, post-mortem DNA decay rapidly reduces the number and quality of surviving DNA fragments, consequently increasing rates of sequencing error and forming a significant obstacle to accurate sequence reconstruction. Recent work has shown that it is possible to engineer a polymerase capable of using even highly damaged fragments as template sequences.

PMID:
18440082
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk