The mutation rate in orientation 1 (OR1, upward black bars) and orientation 2 (OR2, downward gray bars) for each type of error is shown for the hotspot locations (numerical values) and all other sites within the URA3 target (other). When an event was not observed, the upper limit of the mutation rate is shown, labeled with a ≤ symbol above (OR1) or below (OR2) the bar. See Table S1 for mutation rate values.

The mutation rate for each hotspot mutation (in the orientation in which it was hot) is shown for mismatch repair-proficient (pol3-L612M MSH2, white bars, scale on left axis) and deficient (pol3-L612M msh2Δ, black bars, scale on right axis in white font on black background) strains. The observed mutation and URA3 position are shown above each set of bars. The mismatch repair factor (shown below each hotspot mutation) is the ratio of the mutation rates for the two strains. When an event was not observed, the upper limit of the mutation rate is shown, labeled with a ≤ symbol above the bar. See Tables S1 and S3 for mutation rate values.

The orientation (5′ to 3′ with respect to coding sequence) of the URA3 reporter (gray arrow) is depicted by the direction of the arrow. URA3 hotspot locations are identified numerically within the arrow. (A) URA3 orientation 1 (OR1); (B) URA3 orientation 2 (OR2). The lagging (green) and leading (blue) strand templates of the six mutational hotspots are shown, with the hotspot sequence of the preferred templating strand (based on the error bias of L612M Pol δ) shown in bold black font. The observed errors at each site (shown in red above or below the template sequence) are distributed between the leading and lagging strand templates according to the biased error rates of L612M Pol δ determined in vitro using the lacZ forward mutation assay. For the three base substitution hotspots, the mismatch that causes the lagging strand errors is shown in parentheses with the templating base in bold black font and the mismatched incoming nucleotide in red. Mutation rates for lagging strand (green values) and leading strand (blue values) errors for each hotspot were calculated by dividing the number of events assigned to each strand by the total number of events observed in that orientation and then multiplying by the ura3 forward mutation rate of the strain.

Mismatch insertion efficiency of L612M Pol δ was determined in vitro for lagging versus leading strand errors for the URA3 base substitution hotspots at positions (A) 97, (B) 310, and (C) 764. For each panel, the template sequences of the lagging and leading strands are shown on the left and right, respectively, with the site of insertion in white font on a black background. Lanes 1 and 4 of each panel are mock reactions lacking L612M Pol δ; lanes 2 and 5 contain the correct incoming nucleotide (100 μM, 1 min reaction); and lanes 3 and 6 contain the appropriate mismatched nucleotide (1 mM, 10 min reaction). See Table S2 for additional information.

In this model, replication of the leading and lagging strands is performed by Pol ε and Pol δ, respectively. Only the MCM helicase complex is shown at the fork junction, but many other proteins participate in this process. The minimal Okazaki fragment maturation machinery on the lagging strand is shown (see Garg and Burgers, 2005).