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J Biomed Biotechnol. 2007;2007:76514. doi: 10.1155/2007/76514.

Imaging GABAc receptors with ligand-conjugated quantum dots.

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  • 1Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.

Abstract

We report a methodology for labeling the GABAc receptor on the surface membrane of intact cells. This work builds upon our earlier work with serotonin-conjugated quantum dots and our studies with PEGylated quantum dots to reduce nonspecific binding. In the current approach, a PEGylated derivative of muscimol was synthesized and attached via an amide linkage to quantum dots coated in an amphiphilic polymer derivative of a modified polyacrylamide. These conjugates were used to image GABA(C) receptors heterologously expressed in Xenopus laevis oocytes.

PMID:
18437227
[PubMed]
PMCID:
PMC2323551
Free PMC Article

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