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University of Minnesota, Minneapolis, Minnesota, USA.
Stem cells are not only a promising in vivo tool for the treatment of diseases characterized by irreversible tissue damage, but can also be exploited as in vitro systems to study the conditions required to generate molecularly and functionally defined cell types. Constructing functional arteries with luminal arterial endothelial cells stabilized by a medial layer of smooth muscle cells is one of the challenges of regenerative medicine. This unit describes the conditions for generating endothelial and smooth muscle cells from multipotent adult progenitor cells (MAPCs). It elaborates on the importance of certain parameters, e.g., quality control of the stem cell population used, serum lot variations, seeding density, use of appropriate cytokines, critical to obtaining high differentiation efficiencies. It further focuses on the molecular and functional characterization of the obtained cell types.
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