Detection assays for HIV proteins. [Curr Protoc Immunol. 2006]

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1: Curr Protoc Immunol. 2006 Jan;Chapter 12:Unit 12.5.

Detection assays for HIV proteins.

Ostrowski MA, Chun TW, Cheseboro B, Stanley SK, Tremblay M.

University of Toronto, Toronto, Canada.

This updated unit describes four assays for detection of HIV proteins including: (1) a quantitative ELISA, (2) a quantitative immunoblotting assay, (3) a qualitative immunofluorescence assay, and (4) a functional assay to measure virus-associated reverse transcriptase activity as an indicator of viral production.

PMID: 18432967 [PubMed - indexed for MEDLINE]

Enzyme-linked immunosorbent assays for the measurement of human immunodeficiency virus, type 1 reverse transcriptase antigen and antibodies. [J Virol Methods. 1993]
Detection and quantitation of human immunodeficiency virus-infected peripheral blood mononuclear cells by flow cytometry. [J Clin Microbiol. 1990]
Comparison of the sensitivities of two non-isotopic reverse transcriptase (RT) assays for human immunodeficiency virus type 1 RT. [J Virol Methods. 1996]
Markers of human immunodeficiency virus infection in high-risk individuals seronegative by first generation enzyme-linked immunosorbent assay. [Dan Med Bull. 1989]
Detection of reverse transcriptase activity by enzyme-linked immunosorbent assay in human immunodeficiency virus type 1. [Acta Med Okayama. 1995]
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