Transfection of lymphoid cells. [Curr Protoc Immunol. 2001]

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1: Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17B.

Transfection of lymphoid cells.

Morrison SL.

University of California Los Angeles, Los Angeles, California, USA.

Using available vectors, a single immunoglobulin chain or both heavy (H) and light (L) chains can be simultaneously transferred to recipient myeloma cells. Several methods can be used to prepare stable transfected lymphoid cells. The Basic Protocol in this unit describes protoplast fusion, which is suitable for certain lymphoid cells but tends to be more cumbersome than electroporation or liposome-mediated transfection, which are described in the two alternate protocols. All three techniques may be adapted for stable transfection of other eukaryotic cell lines.

PMID: 18432681 [PubMed - indexed for MEDLINE]

A new vector for the high level expression of chimeric antibodies in myeloma cells. [J Immunol Methods. 1994]
Transfection by electroporation. [Curr Protoc Cell Biol. 2003]
Expression of an immunoglobulin heavy chain gene transfected into lymphocytes. [Proc Natl Acad Sci U S A. 1984]
Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells. [Proc Natl Acad Sci U S A. 1983]
Differential efficiency of expression of humanized antibodies in transient transfected mammalian cells. [Hybridoma. 1998]
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Transfection of lymphoid cells.

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