Transfection using DEAE-dextran. [Curr Protoc Immunol. 2001]

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1: Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.14. Links

Transfection using DEAE-dextran.

Selden RF.

Transkaryotic Therapies, Cambridge, Massachusetts, USA.

Two protocols for DEAE-dextran transfection of cells are provided in this unit. The Basic Protocol describes a procedure used to transfect adherent cells and the first Alternate Protocol presents a method used to transfect suspension cells. If an increase in transfection efficiency is needed, cells can be treated with chloroquine as described in the second Alternate Protocol.

PMID: 18432677 [PubMed - indexed for MEDLINE]

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[Curr Protoc Immunol. 2001]
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