Metabolic labeling with amino acids. [Curr Protoc Protein Sci. 2001]

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1: Curr Protoc Protein Sci. 2001 May;Chapter 3:Unit 3.7. Links

Metabolic labeling with amino acids.

Bonifacino JS.

National Institute of Child Health and Human Development, NIH, Bethesda, Maryland, USA.

Metabolic labeling techniques are used to study biosynthesis, processing, intracellular transport, secretion, degradation, and physical-chemical properties of proteins. This unit focuses on pulse-labeling and pulse-chase experiments done with [(35)S]-methionine, but gives directions for labeling with other radiolabeled amino acids, and also offers guidance for the safe use and handling of 35S-labeled compounds.

PMID: 18429172 [PubMed - indexed for MEDLINE]

Metabolic labeling with amino acids.
Curr Protoc Mol Biol. 2001 May; Chapter 10:Unit 10.18.

[Curr Protoc Mol Biol. 2001]
Metabolic labeling with amino acids.
Curr Protoc Cell Biol. 2001 May; Chapter 7:Unit 7.1.

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ReviewThe in vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis.
Methods Mol Biol. 1994; 32:67-72.

[Methods Mol Biol. 1994]
Labeling of proteins with [35S]methionine and/or [35S]cysteine in the absence of cells.
Anal Biochem. 1999 Apr 10; 269(1):179-88.

[Anal Biochem. 1999]
ReviewProduction of 35S-labeled proteins in E. coli and their use as molecular probes.
Methods Mol Biol. 1994; 31:389-96.

[Methods Mol Biol. 1994]
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