cDNA library construction from single cells. [Curr Protoc Neurosci. 2002]

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1: Curr Protoc Neurosci. 2002 Aug;Chapter 4:Unit 4.27.

cDNA library construction from single cells.

Kramer PR.

NIH, NINDS, CDNS, Bethesda, Maryland, USA.

Analysis of gene expression or comparing gene expression patterns between cells and/or tissues facilitates the identification of molecules activated by a cellular program or by a particular physiological or pharmacological treatment. This unit presents a protocol for studying these molecules at the level of a single cell.

PMID: 18428559 [PubMed - indexed for MEDLINE]

Use of monoclonal antibodies for expression cloning. [Curr Protoc Immunol. 2001]
High-efficiency identification of genes by functional analysis from a retroviral cDNA expression library. [J Virol. 1994]
cDNA expression cloning in mammalian cells. [Curr Protoc Neurosci. 2001]
Construction of primary and subtracted cDNA libraries from early embryos. [Methods Enzymol. 1993]
Single-cell cDNA library construction using cycling aRNA amplification. [Methods Mol Biol. 2003]
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cDNA library construction from single cells.

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