Basic confocal microscopy. [Curr Protoc Neurosci. 2001]

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1: Curr Protoc Neurosci. 2001 May;Chapter 2:Unit 2.2.

Basic confocal microscopy.

Smith CL.

National Institute of Neurological Disorders and Stroke, Bethesda, Maryland, USA.

Confocal microscopy produces sharp images of structures within relatively thick specimens (up to several hundred microns). It is particularly useful for examining fluorescent specimens. This overview intended to provide background and practical tips needed to get started with confocal microscopy. It begins with a description of the basis of optical sectioning, then discusses various types of confocal microscopes, and concludes with practical guidelines for sample preparation and optimizing image acquisition parameters.

PMID: 18428457 [PubMed - indexed for MEDLINE]

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Adaptive aberration correction in a confocal microscope. [Proc Natl Acad Sci U S A. 2002]
Multicolor laser scanning confocal immunofluorescence microscopy: practical application and limitations. [Methods Cell Biol. 2002]
Basic confocal microscopy. [Curr Protoc Mol Biol. 2008]
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Basic confocal microscopy.

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