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Curr Protoc Neurosci. 2001 May;Appendix 1:Appendix 1M. doi: 10.1002/0471142301.nsa01ms11.

Digestion of DNA with restriction endonucleases.

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  • 1Massachusetts General Hospital, Charleston, Massachusetts, USA.


Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. This appendix describes restriction endonucleases and their properties.

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