Protein truncation test. [Curr Protoc Hum Genet. 2004]

SearchDatabase nameforSearch term

Advanced Search (beta)

Your browser version may not work well with NCBI's Web applications. More information here...

Display Show

All: 1

Review: 0

Click to change filter selection through MyNCBI.

1: Curr Protoc Hum Genet. 2004 Sep;Chapter 9:Unit9.11.Links

Protein truncation test.

Vossen R, den Dunnen JT.

Human and Clinical Genetics, Leiden University Medical Center, Leiden, The Netherlands.

The protein truncation test detects mutations at the protein level that lead to premature translation termination. The method has evolved considerably since is original publication in this manual. This thoroughly revised unit describes what is now the preferred method for performing the protein truncation test. Transcription and translation are performed in separate reactions; during translation, biotin-labeled or N-terminally tagged proteins are synthesized. The translation products are detected on immunoblots via chemiluminescence. An Alternate Protocol using coupled in vitro transcription/translation and radiolabeled proteins is also presented.

PMID: 18428364 [PubMed - indexed for MEDLINE]

Protein truncation test (PTT) to rapidly screen the DMD gene for translation terminating mutations. [Neuromuscul Disord. 1993]
Premature translation termination mutations are efficiently suppressed in a highly conserved region of yeast Ste6p, a member of the ATP-binding cassette (ABC) transporter family. [J Biol Chem. 1994]
Optimized non-radioactive protein truncation test for mutation analysis of the adenomatous polyposis coli (APC) gene. [Clin Chem Lab Med. 1998]
Phage HK022 Nun protein represses translation of phage lambda N (transcription termination/translation repression). [Proc Natl Acad Sci U S A. 2003]
Biotin in vitro translation, nonradioactive detection of cell-free synthesized proteins. [Biotechniques. 1995]
» See all Related Articles...

Display Show

Protein truncation test.

Related Articles