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Wei Sheng Yan Jiu. 2008 Jan;37(1):12-5.

[Study of C-ZnO composite nanoparticles on viability inhibition and DNA damage of mouse embryo fibroblasts].

[Article in Chinese]

Author information

  • 1Institute of Hygiene and Environmental Medicine, Academy of Military Medical Science, Tianjin 300050, China. yanghui813@126.com

Abstract

OBJECTIVE:

To study the cytotoxicity and DNA damage induced by C-ZnO composite nanoparticles in Mouse embryo fibroblasts(MEF) cells.

METHODS:

MEF cells were treated with the suspension of carbon nanoparticles, zinc oxide nanoparticles and composite nanoparticles at the concentrations of 5, 10, 20, 50, 100 microg/ml. Cellular morphology and MTT assay were carried out to evaluate the viability of cells treated with particles for 24 h, 48 h and 72h respectively. Single cell gel electrophoresis (SCGE, comet assay) was performed to assess DNA damage induced by nanoparticles at the doses of 5 microg x ml(-1) and 10 microg x ml(-1).

RESULTS:

Three types of nanoparticles could induce morphologic change and viability inhibition with significant logarithmic time-effect and dose-effect relationship. Median lethal concentrations (IC50) of carbon nanoparticles, zinc oxide nanoparticles and composite nanoparticles were 21.85, 21.94 and 461.10 mcirog/ml respectively. The inhibitory effect on cellular viability of Zinc oxide nanoparticles increased significantly with the dose enhances. The most DNA damage was induced by composite nanoparticles at lower concentrations.

CONCLUSION:

Nanoparticles with different chemical composition induced distinct cytotoxicity and DNA damage in MEF cells. The metal content of composite nanoparticles played an important role in the toxic effect.

PMID:
18421853
[PubMed - indexed for MEDLINE]
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