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Science. 2008 Apr 18;320(5874):362-5. doi: 10.1126/science.1150021.

The chemical genomic portrait of yeast: uncovering a phenotype for all genes.

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  • 1Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA.

Abstract

Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ( approximately 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition.

PMID:
18420932
[PubMed - indexed for MEDLINE]
PMCID:
PMC2794835
Free PMC Article

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