Epidemiol Infect. 2009 Jan;137(1):102-7. Epub 2008 Apr 16.

Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directly from blood samples.

Ali A, Haque A, Haque A, Sarwar Y, Mohsin M, Bashir S, Tariq A.

Source

Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. ahaq_nibge@yahoo.com

Abstract

Classically Salmonella enterica serovar Typhi (S. Typhi) is associated with typhoid, a major health problem in developing countries. However, in recent years S. Paratyphi A and Vi-negative variants of S. Typhi have emerged rapidly. We have developed a nested multiplex PCR targeting five different genes for differential diagnosis of typhoidal pathogens which has been optimized to be directly applicable on clinical blood samples. Of 42 multiplex PCR-positive blood samples, 26, nine, and two were Vi-positive S. Typhi, Vi-negative S. Typhi and S. Paratyphi A, respectively, and five patients were found to have mixed infection. Seventeen patients grew Salmonella from blood culture and the remaining 25 were positive in the Salmonella-specific PCR. Tests with several common pathogens confirmed the specificity of the assay. We conclude that the proposed multiplex PCR is rapid, sensitive and specific for the diagnosis of typhoidal pathogens directly from blood samples.

PMID:: 18413005; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Medical

Blood and Blood Disorders - MedlinePlus Health Information

Supplemental Content

Save items

Related citations in PubMed

PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever. [J Clin Microbiol. 2008]
PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever.
Levy H, Diallo S, Tennant SM, Livio S, Sow SO, Tapia M, Fields PI, Mikoleit M, Tamboura B, Kotloff KL, et al. J Clin Microbiol. 2008 May; 46(5):1861-6. Epub 2008 Mar 26.
The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens. [BMC Infect Dis. 2010]
The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens.
Nga TV, Karkey A, Dongol S, Thuy HN, Dunstan S, Holt K, Tu le TP, Campbell JI, Chau TT, Chau NV, et al. BMC Infect Dis. 2010 May 21; 10:125. Epub 2010 May 21.
Reliable means of diagnosis and serovar determination of blood-borne Salmonella strains: quick PCR amplification of unique genomic loci by novel primer sets. [J Clin Microbiol. 2009]
Reliable means of diagnosis and serovar determination of blood-borne Salmonella strains: quick PCR amplification of unique genomic loci by novel primer sets.
Nagarajan AG, Karnam G, Lahiri A, Allam US, Chakravortty D. J Clin Microbiol. 2009 Aug; 47(8):2435-41. Epub 2009 Jun 17.
Review Pancreatitis in enteric fever. [Indian J Gastroenterol. 2002]
Review Pancreatitis in enteric fever.
Kadappu KK, Rao PV, Srinivas N, Shastry BA. Indian J Gastroenterol. 2002 Jan-Feb; 21(1):32-3.
Review Recent advances in the diagnosis of enteric fever. [Trop Gastroenterol. 1995]
Review Recent advances in the diagnosis of enteric fever.
Chaudhry R, Shankar S, Nisar N, Dey AB. Trop Gastroenterol. 1995 Jul-Sep; 16(3):8-12.

See reviews... See all...

Cited by 4 PubMed Central articles

Ultra-fast and sensitive detection of non-typhoidal Salmonella using microwave-accelerated metal-enhanced fluorescence ("MAMEF"). [PLoS One. 2011]
Ultra-fast and sensitive detection of non-typhoidal Salmonella using microwave-accelerated metal-enhanced fluorescence ("MAMEF").
Tennant SM, Zhang Y, Galen JE, Geddes CD, Levine MM. PLoS One. 2011 Apr 8; 6(4):e18700. Epub 2011 Apr 8.
The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens. [BMC Infect Dis. 2010]
The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens.
Nga TV, Karkey A, Dongol S, Thuy HN, Dunstan S, Holt K, Tu le TP, Campbell JI, Chau TT, Chau NV, et al. BMC Infect Dis. 2010 May 21; 10:125. Epub 2010 May 21.
A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar Typhi. [Ann Clin Microbiol Antimicrob....]
A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar Typhi.
Zhou L, Pollard AJ. Ann Clin Microbiol Antimicrob. 2010 Apr 19; 9:14. Epub 2010 Apr 19.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directl...
Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directly from blood samples.
Epidemiol Infect. 2009 Jan ;137(1):102-7. Epub 2008 Apr 16 .

PubMed
Nutrigenetics and CVD: what does the future hold?
Nutrigenetics and CVD: what does the future hold?
Proc Nutr Soc. 2008 May ;67(2):206-13.

PubMed
Improving protein function prediction methods with integrated literature data.
Improving protein function prediction methods with integrated literature data.
BMC Bioinformatics. 2008 Apr 15 ;9:198.

PubMed
Reliability of medical students' vaccination histories for immunisable diseases.
Reliability of medical students' vaccination histories for immunisable diseases.
BMC Public Health. 2008 Apr 15 ;8:121.

PubMed
Quantifying the effectiveness of climate change mitigation through forest planta...
Quantifying the effectiveness of climate change mitigation through forest plantations and carbon sequestration with an integrated land-use model.
Carbon Balance Manag. 2008 Apr 15 ;3:3.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directly from blood samples.

Source

Abstract

Publication Types, MeSH Terms

Publication Types

MeSH Terms

LinkOut - more resources

Full Text Sources

Medical

Supplemental Content

Save items

Related citations in PubMed

Cited by 4 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information