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FEBS Lett. 2008 Apr 30;582(10):1564-8. doi: 10.1016/j.febslet.2008.03.057. Epub 2008 Apr 10.

Downregulation of CCND1 and CDK6 by miR-34a induces cell cycle arrest.

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  • 1Beijing Institute of Radiation Medicine, Beijing, People's Republic of China.

Abstract

miRNAs regulate gene expression by inhibiting translation or by targeting messenger RNA (mRNA) for degradation in a post-transcriptional fashion. In the present study, we show that ectopic expression of miR-34a reduces both mRNA and protein levels of cyclin D1 (CCND1) and cyclin-dependent kinase 6 (CDK6). We also demonstrate that miR-34a targets the 3'-untranslated mRNA region of CCND1 as well as CDK6, which in turn interferes with phosphorylation of retinoblastoma. In addition, we show that overexpression of miR-34a induces a significant G1 cell-cycle arrest in the A549 cell line. Taken together, our data suggest that the effects of miR-34a on G1 cell cycle arrest are through the down-regulation of CCND1 and CDK6, which is associated with other targets of miR-34a either additively or synergistically.

PMID:
18406353
[PubMed - indexed for MEDLINE]
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