Nucleotide sequence of the 3' non-coding region and N gene of the S RNA of a serologically distinct tospovirus

J Gen Virol. 1991 Oct:72 ( Pt 10):2597-601. doi: 10.1099/0022-1317-72-10-2597.

Abstract

A tomato spotted wilt-like virus (TSWV-I) is a distinct member of the Tospovirus genus of the Bunyaviridae and is distinguished from the typical TSWV by having a serologically distinct nucleoprotein (N). A cDNA clone extending from the 3' terminus of the viral RNA through the entire N open reading frame (ORF) was sequenced. The TSWV-I N ORF is capable of encoding a polypeptide of 262 amino acids with a predicted Mr of 28.8K. In vitro transcription and translation of the clone produced a protein which comigrated with TSWV-I N and was immunoprecipitated by TSWV-I antibodies. Hybridization analysis of lithium chloride-precipitated RNA from healthy and TSWV-I-infected tissue detected a virus-specific 1.2 kb subgenomic RNA. The TSWV-I S RNA terminal consensus sequence (8 nucleotides) was identical to that of TSWV; the remaining TSWV-I untranslated region showed only 51% identity with that of TSWV. Comparison of the TSWV-I and TSWV N proteins showed 67% identity at the amino acid level. The degree of similarity in the terminal sequence, untranslated region and N ORF is similar to that expected between distinct serogroups within certain genera of the Bunyaviridae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Bunyaviridae / genetics*
  • Capsid / genetics*
  • Genes, Viral
  • Introns*
  • Molecular Sequence Data
  • Open Reading Frames
  • RNA, Viral / genetics*
  • Sequence Alignment
  • Viral Core Proteins / genetics*

Substances

  • RNA, Viral
  • Viral Core Proteins

Associated data

  • GENBANK/D00914
  • GENBANK/D00931
  • GENBANK/D00932
  • GENBANK/D10545
  • GENBANK/M59339
  • GENBANK/M59340
  • GENBANK/M59341
  • GENBANK/M59342
  • GENBANK/M59343
  • GENBANK/M59344