Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Insect Mol Biol. 2008 Jun;17(3):217-25. doi: 10.1111/j.1365-2583.2008.00797.x. Epub 2008 Apr 7.

Cloning and molecular characterization of two invertebrate-type lysozymes from Anopheles gambiae.

Author information

  • 1Department of Entomology, University of Wisconsin, Madison, Wisconsin, USA.

Abstract

We sequenced and characterized two novel invertebrate-type lysozymes from the mosquito Anopheles gambiae. Alignment and phylogenetic analysis of these and a number of related insect proteins identified through bioinformatics strategies showed a high degree of conservation of this protein family throughout the Class Insecta. Expression profiles were examined for the two mosquito genes through semiquantitative and real-time PCR analysis. Lys i-1 transcripts were found in adult females in the fat body and Malpighian tubules, whereas Lys i-2 was detected only in fat bodies. Blood-feeding resulted in significantly increased transcript abundance for both genes in the midguts. Neither gene was upregulated following bacterial challenge.

PMID:
18397277
[PubMed - indexed for MEDLINE]
PMCID:
PMC2995370
Free PMC Article

Images from this publication.See all images (7)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Blackwell Publishing Icon for PubMed Central
    Loading ...
    Write to the Help Desk