a–c wingless (wg)/Wnt1. a In the germ rudiment, wg is expressed in the head lobes and posteriorly in a subterminal position that marks the developing hindgut and leaves the posterior pole free of expression (arrow). b In the extending germband embryo, stripes of wg are visible in the antennal, gnathal and thoracic segments anterior to the growth zone. c wg expression extends ventrally into the appendages of the fully extended germband embryo. In the head wg expression appears in the labrum and the developing brain lobes. wg is also expressed in patches along the lateral edge of each segment and in the developing hindgut.
d–f Wnt5. d Wnt5 is expressed faintly in the head lobes (arrow) and in segmental stripes in an embryo beginning germband elongation. More intense expression is visible at the posterior end of the growth zone. e Stripes of Wnt5 in the gnathal and thoracic segments cross the ventral midline. Wnt5 expression is more intense at the posterior end and in broad wedge shaped domains in the head lobes. f In the fully extended germband embryo, Wnt5 is no longer expressed at the posterior end of the growth zone, but extends ventrally into the developing limbs and is also visible at their tips.
g–i Wnt6. g In an embryo beginning germband elongation, Wnt6 is expressed faintly in the lateral head lobes (arrow), in segmental stripes and in a subterminal domain in the growth zone (arrowhead). h After elongation, a single segmental stripe remains, marking the eleventh abdominal segment, while expression is observed along the ventral edge of the limbs, along the dorsolateral edge of the embryo and in spots to the left and right of the midline. Expression near the posterior end of the germband marks the anlagen of the Malpighian tubules (arrowhead). i In the retracted germband, fore- and hindgut anlagen express Wnt6, as do precursors of the tracheal openings. Several spots of Wnt6 expression are also evident in the brain. Non-specific expression in the pleuropodia is marked by an asterisk.
j–l Wnt7. j Faint segmental stripes of Wnt7 are apparent in the elongating embryo. k In a slightly older embryo, gnathal expression of Wnt7 is restricted to specific cells in the developing CNS, while in the thorax, expression encompasses the leg anlagen. l In the thorax and abdomen of the germband extended embryo, segmental stripes of Wnt7, which extend laterally into the dorsal mesoderm of the appendages, do not cross the midline. m Wnt8/D. During elongation, Wnt8/D remains expressed at the posterior end of the growth zone in two small patches, one at each side of the ventral mesoderm, that are connected by a narrow row of cells at a subterminal position.
n–p Wnt9 n In late stage embryos Wnt9 expression is visible in cells of the fore- and hindgut that appear to be positioned at the border of the ectodermal and endodermal derivatives of the gut. o Closeup view of the embryo in n focusing on the foregut (arrow). p Closeup view of Wnt9 expression at the anterior end of the hindgut, where it connects to the midgut (arrow). Nonspecific staining of the pleuropodia in abdominal segment 1 is marked by an asterisk.
q–s Wnt10. q Wnt10 expression is first visible in a single stripe in the mandibular segment. r In elongated embryos segmental stripes have resolved into dots of expression that extend ventrally into the developing limbs. Three prominent cells in each hemisphere of the developing brain also begin to express Wnt10. s During retraction, segmental expression of Wnt10 remains in the ventral limbs and in a few cells on either side of the ventral midline while expression in the brain has expanded.
t–v Wnt11. t Wnt11 expression is visible in cells of the serosa, remnants of which are evident along the edge of the embryo and covering part of the posterior end. u In the germ-band extended embryo, Wnt11expression is visible in the distal tips of the developing legs. The distal regions of the maxillary and labial appendages express Wnt11 strongly but it is seen only weakly in the antennal lobes and there is no expression in the mandibles (arrowhead). Wnt11 is also expressed at the border of the dorsal ectoderm (arrow). v As the legs mature during germband retraction, expression of Wnt11 there resolves proximally into rings and distally into a ventral patch. Only a few antennal and labral cells express Wnt11, while expression is strong in the anal plate.
w–y WntA. w In a germband embryo beginning elongation, WntA is expressed faintly in lateral domains of the head lobes, segmentally and in the growth zone. x Segmental expression appears as stripes only in the most posterior abdominal segments where it resolves into spots on either side of the ventral midline (arrows). As elongation nears completion, expression in the growth zone fades, while head specific expression develops. y During germband retraction, WntA expression appears in the developing hindgut and in the labrum. Expression also appears in the limbs in a ring at midlength and in patches along the lateral edge of the embryo.