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1: Vet Parasitol. 2008 Jun 14;154(1-2):148-50. Epub 2008 Feb 29.Click here to read Links

Infectivity of Hepatozoon americanum cystozoites for a dog.

Johnson EM, Allen KE, Panciera RJ, Little SE, Ewing SA.

Oklahoma State University (OSU), College of Veterinary Health Sciences (CVHS), Department of Veterinary Pathobiology, Room 250 McElroy Hall, Stillwater, OK 74078, United States. eileen.johnson@okstate.edu

Hepatozoon americanum cystozoites from experimentally infected, laboratory-raised rodents were fed to a Hepatozoon-free dog. Gamonts were detected by examination of blood smear 42 and 56 days post-exposure. PCR analysis of blood was positive for the 18S rRNA Hepatozoon gene on days gamonts were demonstrated. Meronts were detected histologically in a skeletal muscle biopsy 90 days after ingestion of cystozoites. Sequencing confirmed that the parasite in the dog was H. americanum. Xenodiagnosis was conducted by replete feeding of Ambylomma maculatum larvae on the dog; 40 days after detachment, sporulated oocysts were recovered from recently molted nymphs.

PMID: 18387744 [PubMed - indexed for MEDLINE]