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Appl Microbiol Biotechnol. 2008 Jun;79(3):471-9. doi: 10.1007/s00253-008-1444-z. Epub 2008 Apr 1.

Corynebacterium glutamicum tailored for high-yield L-valine production.

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  • 1Institute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany.

Abstract

We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested.

PMID:
18379776
[PubMed - indexed for MEDLINE]
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