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1: Methods Mol Biol. 2008;439:291-308.Click here to read Links

Coimmunoprecipitation and proteomic analyses.

Falsone SF, Gesslbauer B, Kungl AJ.

Institute of Pharmaceutical Sciences, University of Graz, Graz, Austria.

Defining protein-protein interaction networks is a major goal of proteomics. Here, we present a protocol for coimmunoprecipitation, a technique suitable for the isolation of whole protein complexes in vivo and their subsequent identification by either immunoblotting or mass spectrometric sequencing combined to database search.

PMID: 18370111 [PubMed - indexed for MEDLINE]