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Coimmunoprecipitation and proteomic analyses.
Institute of Pharmaceutical Sciences, University of Graz, Graz, Austria.
Defining protein-protein interaction networks is a major goal of proteomics. Here, we present a protocol for coimmunoprecipitation, a technique suitable for the isolation of whole protein complexes in vivo and their subsequent identification by either immunoblotting or mass spectrometric sequencing combined to database search.
PMID: 18370111 [PubMed - indexed for MEDLINE]
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