Biotechniques. 2008 Mar;44(3):413-6.

Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts.

Pacholczyk G, Suhag R, Mazurek M, Dederscheck SM, Koni PA.

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Mouse Embryonic Stem Cell & Transgenesis Core Laboratories, Medical College of Georgia, Augusta, GA, USA.

Abstract

The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F1 blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.

PMID:: 18361795; [PubMed - indexed for MEDLINE]

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Cited by 2 PubMed Central articles

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Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts.

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