Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
ACS Chem Biol. 2008 Mar 20;3(3):156-60. doi: 10.1021/cb700247q.

Simultaneous recording of multiple cellular events by FRET.

Abstract

The function of many sensors for measuring intracellular events is based on Förster resonance energy transfer (FRET). Here we demonstrate for the first time the use of multiple ratiometric FRET sensors in parallel through spatial and spectral resolution. We monitored three calcium-dependent signaling events by a cytosolic sensor for calcium/calmodulin-dependent protein kinase IIalpha, a membrane-bound sensor for protein kinase C, and a translocating FRET probe based on annexin A4. This multiparameter imaging approach gives insight into the precise timing of cellular events within one single cell, thereby providing a major advantage over single-parameter protocols. This type of imaging will likely be important for high content cell analysis and screening efforts in the future.

PMID:
18355004
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Write to the Help Desk