Comparing whole genomes using DNA microarrays.

Lewis-Sigler Institute for Integrative Genomics, Department of Molecular Biology, Carl Icahn Laboratory, Princeton University, Princeton, New Jersey 08544, USA. dgresham@genomics.princeton.edu

The rapid accumulation of complete genomic sequences offers the opportunity to carry out an analysis of inter- and intra-individual genome variation within a species on a routine basis. Sequencing whole genomes requires resources that are currently beyond those of a single laboratory and therefore it is not a practical approach for resequencing hundreds of individual genomes. DNA microarrays present an alternative way to study differences between closely related genomes. Advances in microarray-based approaches have enabled the main forms of genomic variation (amplifications, deletions, insertions, rearrangements and base-pair changes) to be detected using techniques that are readily performed in individual laboratories using simple experimental approaches.

PMID: 18347592 [PubMed - indexed for MEDLINE]