J Biol Chem. 1991 Nov 25;266(33):22380-5.

Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine.

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Section of Microbiology, Cornell University, Ithaca, New York 14853-8101.

Abstract

The fdnGHI operon of Escherichia coli encodes nitrate-inducible formate dehydrogenase. We report here the entire nucleotide sequence of fdnGHI. The sequence contains three open reading frames of sizes appropriate to encode the three subunits of formate dehydrogenase-N. fdnG contains an in-frame UGA codon that specifies selenocysteine incorporation, and the predicted amino acid sequence of FdnG shows similarity to two other bacterial formate dehydrogenase enzymes. FdnH contains 4 cysteine clusters typical of those found in iron-sulfur proteins. FdnG also contains a cysteine cluster. Evidence from sequence and spectral analyses suggest that FdnI encodes cytochrome bFdn556. Implications for the membrane topology of formate dehydrogenase-N and its mechanism of proton translocation are discussed.

PMID:: 1834669; [PubMed - indexed for MEDLINE]

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Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. II. Evidence that a mRNA stem-loop structure is essential for decoding opal (UGA) as selenocysteine. [J Biol Chem. 1991]
Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. II. Evidence that a mRNA stem-loop structure is essential for decoding opal (UGA) as selenocysteine.
Berg BL, Baron C, Stewart V. J Biol Chem. 1991 Nov 25; 266(33):22386-91.
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Zinoni F, Heider J, Böck A. Proc Natl Acad Sci U S A. 1990 Jun; 87(12):4660-4.
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Cited by 38 PubMed Central articles

Selective selC-Independent Selenocysteine Incorporation into Formate Dehydrogenases. [PLoS One. 2013]
Selective selC-Independent Selenocysteine Incorporation into Formate Dehydrogenases.
Zorn M, Ihling CH, Golbik R, Sawers RG, Sinz A. PLoS One. 2013; 8(4):e61913. Epub 2013 Apr 25.
The formate channel FocA exports the products of mixed-acid fermentation. [Proc Natl Acad Sci U S A. 2012]
The formate channel FocA exports the products of mixed-acid fermentation.
Lü W, Du J, Schwarzer NJ, Gerbig-Smentek E, Einsle O, Andrade SL. Proc Natl Acad Sci U S A. 2012 Aug 14; 109(33):13254-9. Epub 2012 Jul 30.
A-type carrier protein ErpA is essential for formation of an active formate-nitrate respiratory pathway in Escherichia coli K-12. [J Bacteriol. 2012]
A-type carrier protein ErpA is essential for formation of an active formate-nitrate respiratory pathway in Escherichia coli K-12.
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Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide ...
Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine.
J Biol Chem. 1991 Nov 25 ;266(33):22380-5.

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