Detection and quantitation of Solenopsis invicta virus-2 genomic and intermediary replicating viral RNA in fire ant workers and larvae

Yoshifumi Hashimoto; Steven M Valles

doi:10.1016/j.jip.2008.02.002

Detection and quantitation of Solenopsis invicta virus-2 genomic and intermediary replicating viral RNA in fire ant workers and larvae

J Invertebr Pathol. 2008 Jun;98(2):243-5. doi: 10.1016/j.jip.2008.02.002. Epub 2008 Feb 13.

Authors

Yoshifumi Hashimoto¹, Steven M Valles

Affiliation

¹ Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Drive, Gainesville, FL 32608, USA.

PMID: 18343402
DOI: 10.1016/j.jip.2008.02.002

Abstract

Quantitative real-time PCR (QPCR) was used to quantify the genome of Solenopsis invicta virus-2 (SINV-2) from infected individual ants of S. invicta. Strand-specific cDNA synthesis oligonucleotide primers and RNase digestion after cDNA synthesis allowed quantification of plus (genomic) and minus (replicative) strands of the SINV-2 genome. Both strands were detected in adult workers and larval fire ants indicating that the virus was replicating within the ant. The differences between the genomic to replicative strand ranged from 199-fold in larvae to 479-fold in workers with an average ratio of 339:1.

MeSH terms

Animals
Ants / virology*
Genes, Viral*
Genome, Viral*
Insect Viruses / genetics*
Larva / virology
RNA Viruses / genetics*
RNA, Viral / analysis
RNA, Viral / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Virus Replication

Substances

RNA, Viral