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    Stroke. 2008 May;39(5):1613-5. Epub 2008 Mar 13.

    Middle cerebral artery occlusion in the rabbit using selective angiography: application for assessment of thrombolysis.

    Abstract

    BACKGROUND AND PURPOSE:

    An animal model of selective middle cerebral artery (MCA) occlusion is needed for evaluation of intra-arterial (IA) delivery of thrombolytic agents. We describe a technique for MCA thrombo-occlusion in the rabbit with real-time angiographic documentation of occlusion and thrombolytic recanalization.

    METHODS:

    After femoral artery cutdown, a microcatheter was advanced from the internal carotid artery to the MCA. MCA occlusion was achieved by IA thrombin and reperfusion by IA plasmin.

    RESULTS:

    The terminal internal carotid artery was successfully catheterized in 12 of 13 animals. Stable (2-hour) MCA occlusion was induced and verified angiographically in all 12 animals; 2 animals also had distal internal carotid artery thrombus. Recanalization was achieved rapidly after IA plasmin in 3 of 3 animals.

    CONCLUSIONS:

    We describe a new animal model of selective MCA occlusion documented by real-time angiography and used to demonstrate recanalization with IA plasmin.

    PMID:
    18340097
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC2443943
    Free PMC Article

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