Stochastic rotational catalysis of proton pumping F-ATPase.

Futai Special Laboratory, Microbial Chemistry Research Foundation, Tokyo 141-0021, Japan Department of Biochemistry, Faculty of Pharmaceutical Sciences, Iwate Medical University, Iwate 028-3694, Japan.

F-ATPases synthesize ATP from ADP and phosphate coupled with an electrochemical proton gradient in bacterial or mitochondrial membranes and can hydrolyse ATP to form the gradient. F-ATPases consist of a catalytic F1 and proton channel F0 formed from the alpha3beta3gammadelta and ab2c10 subunit complexes, respectively. The rotation of gammac10 couples catalyses and proton transport. Consistent with the threefold symmetry of the alpha3beta3 catalytic hexamer, 120 degrees stepped revolution has been observed, each step being divided into two substeps. The ATP-dependent revolution exhibited stochastic fluctuation and was driven by conformation transmission of the beta subunit (phosphate-binding P-loop/alpha-helix B/loop/beta-sheet4). Recent results regarding mechanically driven ATP synthesis finally proved the role of rotation in energy coupling.

PMID: 18339602 [PubMed - as supplied by publisher]