Format

Send to:

Choose Destination
See comment in PubMed Commons below
Dev Cell. 2008 Mar;14(3):331-41. doi: 10.1016/j.devcel.2007.12.007.

sSgo1, a major splice variant of Sgo1, functions in centriole cohesion where it is regulated by Plk1.

Author information

  • 1Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY 10987, USA.

Abstract

Shugoshin 1 (Sgo1) functions as a protector of centromeric cohesion of sister chromatids in higher eukaryotes. Here, we provide evidence for a previously unrecognized role for Sgo1 in centriole cohesion. Sgo1 depletion via RNA interference induces the formation of multiple centrosome-like structures in mitotic cells that result from the separation of paired centrioles. Sgo1+/- mitotic murine embryonic fibroblasts display split centrosomes. Localization study of two major endogenous splice variants of Sgo1 indicates that the smaller variant, sSgo1, is found at the centrosome in interphase and at spindle poles in mitosis. sSgo1 interacts with Plk1 and its spindle pole localization is Plk1 dependent. Centriole splitting induced by Sgo1 depletion or expression of a dominant negative mutant is suppressed by ectopic expression of sSgo1 or by Plk1 knockdown. Our studies strongly suggest that sSgo1 plays an essential role in protecting centriole cohesion, which is partly regulated by Plk1.

Comment in

PMID:
18331714
[PubMed - indexed for MEDLINE]
PMCID:
PMC2279080
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk