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Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Apr;105(4):526-9. doi: 10.1016/j.tripleo.2007.11.005.

Observations of biofilm growth on human dentin and potential destruction after exposure to antibiotics.

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  • 1Department of Endodontics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.

Abstract

OBJECTIVES:

This study was performed to observe biofilm formation on dentin and to then observe effects of clinically achievable antimicrobial drug concentrations on these biofilms.

STUDY DESIGN:

Wild-strain endodontic bacteria were anaerobically cultured from necrotic pulps of extracted human teeth and used to grow biofilms on sterilized dentin slices in an anaerobic chamber for 12 days. Then these biofilms were exposed to ampicillin, doxycycline, clindamycin, azithromycin, or metronidazole. Each day for 8 days, specimens were fixed using 2% glutaraldehyde and examined with a scanning electron microscope (SEM).

RESULTS:

The SEM images revealed the presence of a mature biofilm after 8 days of growth and that none of the antibiotics tested was effective in eliminating the biofilm even after 8 days of exposure.

CONCLUSION:

Biofilms are formed in a few days and are resistant to antimicrobial drugs.

PMID:
18329587
[PubMed - indexed for MEDLINE]
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