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Nat Methods. 2008 Apr;5(4):299-302. doi: 10.1038/nmeth.1190. Epub 2008 Mar 9.

Genetic control of neuronal activity in mice conditionally expressing TRPV1.

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  • 1Howard Hughes Medical Institute, Department of Neurobiology, Duke University Medical Center, Box 3209, Durham, North Carolina 27710, USA.


Here we describe a knock-in mouse model for Cre-loxP-based conditional expression of TRPV1 in central nervous system neurons. Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered heterologous expression of TRPV1 in a cell-specific manner. Application of the TRPV1 ligand capsaicin induced strong inward currents, triggered action potentials and activated stereotyped behaviors, allowing cell type-specific chemical genetic control of neuronal activity in vitro and in vivo.

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