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Nat Methods. 2008 Apr;5(4):315-8. doi: 10.1038/nmeth.1192. Epub 2008 Mar 9.

Identification of cross-linked peptides from large sequence databases.

Author information

  • 1Institute of Molecular Systems Biology, Eidgenössische Technische Hochschule (ETH) Zurich, Wolfgang-Pauli Strasse 16, 8093 Zurich, Switzerland.

Erratum in

  • Nat Methods. 2008 Aug;5(8):748.

Abstract

We describe a method to identify cross-linked peptides from complex samples and large protein sequence databases by combining isotopically tagged cross-linkers, chromatographic enrichment, targeted proteomics and a new search engine called xQuest. This software reduces the search space by an upstream candidate-peptide search before the recombination step. We showed that xQuest can identify cross-linked peptides from a total Escherichia coli lysate with an unrestricted database search.

PMID:
18327264
[PubMed - indexed for MEDLINE]
PMCID:
PMC2719781
Free PMC Article
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