-
Engineering Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system.
Massachusetts General Hospital, Charlestown, USA.
Synthetic Cys2His2 zinc finger domains with novel DNA-binding specificities can be identified from large randomized libraries using selection methodologies such as phage display. It has been previously demonstrated that a bacterial cell-based two-hybrid system is at least as effective as phage display for selecting zinc fingers with desired specificities from these libraries. In this chapter the authors provide updated and detailed protocols for performing zinc finger selections using the bacterial two-hybrid system.
PMID: 18314590 [PubMed - indexed for MEDLINE]
-
Cited by 2 PubMed Central articles
-
Rapid mutation of endogenous zebrafish genes using zinc finger nucleases made by Oligomerized Pool ENgineering (OPEN).
Foley JE, Yeh JR, Maeder ML, Reyon D, Sander JD, Peterson RT, Joung JK.
PLoS One. 2009; 4(2):e4348. Epub 2009 Feb 9.
[PLoS One. 2009]
-
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, et al.
Mol Cell. 2008 Jul 25; 31(2):294-301.
[Mol Cell. 2008]