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    Physiol Plant. 2008 Jun;133(2):117-30. Epub 2008 Feb 25.

    Functional genomics in a non-model crop: transcriptomics or proteomics?

    Carpentier SC, Coemans B, Podevin N, Laukens K, Witters E, Matsumura H, Terauchi R, Swennen R, Panis B.

    Source

    Laboratory of Tropical Crop Improvement, Katholieke Universiteit, 3001 Leuven, Belgium. sebastien.carpentier@biw.kuleuven.be

    Abstract

    There is no question that protein- and RNA-based measurements are complementary, but which approach has the highest return in the case of a non-model crop and what is the correlation between mRNA and proteins? We describe and evaluate in detail the advantages and pitfalls of both a proteomics and a transcriptomics approach. The information on the abundance of transcripts was obtained by serial analysis of gene expression (SAGE), while information on the abundance of proteins was obtained via two-dimensional gel electrophoresis.

    PMID:
    18312499
    [PubMed - indexed for MEDLINE]

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