J Chromatogr A. 2008 Apr 11;1187(1-2):205-8. Epub 2008 Feb 14.

Increasing voltage gradient electrophoresis of DNA.

Oliva D, Di Maio S, Sisino G, Bellavia D, Barbieri R.

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Istituto di Biomedicina e Immunologia Molecolare del CNR, Palermo, Italy.

Abstract

We developed a method which allows electrophoretic fractionation of DNA in an agarose matrix according to an increasing current gradient, using a previously designed [R. Barbieri, V. Izzo, M.A. Costa, G. Giudice, G. Duro, Anal. Biochem. 212 (1993) 168; M.R. Asaro, V. Izzo, R. Barbieri, J. Chromatogr. A 855 (1999) 723] voltage gradient apparatus. This method allows the separation of different DNA fragments by increasing the distances of the components fractionated in the gel, revealing small differences in the length of different DNA components.

PMID:: 18304558; [PubMed - indexed for MEDLINE]

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