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Genome Biol. 2008;9(2):R43. Epub 2008 Feb 26.

UTILLdb, a Pisum sativum in silico forward and reverse genetics tool.

Dalmais M, Schmidt J, Le Signor C, Moussy F, Burstin J, Savois V, Aubert G, Brunaud V, de Oliveira Y, Guichard C, Thompson R, Bendahmane A.

Source

Unité de Recherche en Génomique Végétale, UMR INRA-CNRS, Rue Gaston Crémieux, 91057 Evry Cedex, France. dalmais@evry.inra.fr

Abstract

The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords.

PMID:: 18302733; [PubMed - indexed for MEDLINE]
PMCID:: PMC2374714

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ETHYL METHANESULFONATE - HSDB

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TILLING in Lotus japonicus identified large allelic series for symbiosis genes and revealed a bias in functionally defective ethyl methanesulfonate alleles toward glycine replacements. [Plant Physiol. 2009]
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Review Tomato mutants as tools for functional genomics. [Curr Opin Plant Biol. 2002]
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Cited by 14 PubMed Central articles

Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers. [BMC Genomics. 2012]
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Review TILLING: a shortcut in functional genomics. [J Appl Genet. 2011]
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