PURPOSE: Mentoring is critical to develop and nurture early career investigators, helping them to succeed in building networks of colleagues, and is especially important for investigators focused on HIV research. We piloted a multidiscipline mentoring program targeting postdoctoral scholars and early career faculty concentrating on HIV/AIDS research. METHOD: The pilot mentoring program was conducted at the Center for AIDS Research (CFAR) at the University of California San Francisco and the Gladstone Institute of Virology and Immunology. Mentees were self-referred postdoctoral scholars and early career faculty. Mentors were drawn from the senior faculty. Early career mentees were matched with senior investigators for individual meetings, a monthly workshop on topics directed by the mentees, and single-day mentoring seminars. RESULTS: More than 30 mentees and 20 mentors have participated in the pilot project. Most mentees reported that the 1-on-1 mentoring was a satisfying experience. The most highly valued activities were those that facilitated networking among mentees, networking between mentors and mentees, and workshops that focused on grant applications and first academic appointments and promotions. CONCLUSIONS: A multidisciplinary mentoring program for postdoctoral scholars and early career faculty focused on HIV/AIDS research is valuable. Umbrella organizations, such as the CFAR, are well suited to create and provide highly valued mentoring experiences.