Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Anal Chem. 2008 Mar 15;80(6):1962-6. doi: 10.1021/ac7019915. Epub 2008 Feb 21.

Enhanced fluorescent immunoassays on silver fractal-like structures.

Author information

  • 1Department of Chemistry, The University of Texas at Tyler, 3900 University Boulevard, Tyler, Texas 75799, USA. tshtoyko@uttyler.edu

Abstract

Using the effect of the fluorescence enhancement in close proximity to metal nanostructures, we have been able to demonstrate ultrasensitive immunoassays suitable for the detection of biomarkers. Silver fractal-like structures have been grown by electrochemical reduction of silver on the surface of glass slides. A model immunoassay was performed on the slide surface with rabbit IgG (antigen) noncovalently immobilized on the slide, and rhodamine red-X-labeled antirabbit IgG conjugate was subsequently bound to the immobilized antigen. The fluorescence signal was measured from the glass-fractal's surface using a confocal microscope, and the images were compared to the images from the same surface not coated with fractals. Our results showed significant enhancement (more than 100-fold) of the signal detected on fractals compared to bare glass. We thus demonstrate that such fractal-like structures can assist in improving the signals from assays used in medical diagnostics, especially those for analytes with molecular weight under 100 kDa.

PMID:
18288816
[PubMed - indexed for MEDLINE]
PMCID:
PMC2734186
Free PMC Article

Images from this publication.See all images (6)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Write to the Help Desk