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    J Biol Chem. 1991 Jun 25;266(18):11669-75.

    Structure of the rabbit phospholamban gene, cloning of the human cDNA, and assignment of the gene to human chromosome 6.

    Fujii J, Zarain-Herzberg A, Willard HF, Tada M, MacLennan DH.

    Source

    Banting and Best Department of Medical Research, Charles H. Best Institute, University of Toronto, Ontario, Canada.

    Abstract

    We have isolated and characterized genomic DNA clones encoding rabbit phospholamban. Only a single gene for phospholamban was detected in the rabbit genome. The phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. Two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mRNA or 239 and 89 nucleotides upstream from the exon/intron boundary. Phospholamban gene expression in various smooth muscle tissues, as well as in cardiac and slow twitch skeletal muscle, was detected by Northern blotting. The phospholamban gene was mapped to human chromosome 6, using a human phospholamban cDNA.

    PMID:
    1828805
    [PubMed - indexed for MEDLINE]
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