Send to:

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2008;431:213-21.

Determining the cellular targets of reactive oxygen species in Borrelia burgdorferi.

Author information

  • 1Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratoties, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.


The response of Borrelia burgdorferi to the challenge of reactive oxygen species (ROS) is a direct result of its limited biosynthetic capabilities and lack of biologically significant levels of intracellular Fe. In other bacteria, the major target for oxidative damage is DNA as a consequence of the reaction of "free" intracellular with ROS through the Fenton reaction. Therefore, cellular defenses in these bacteria are focused on protecting this essential cellular component. This does not seem to be the case for B. burgdorferi. In this chapter, we describe methods that were used to analyze the potential targets for ROS in B. burgdorferi. Surprisingly, membrane lipids (e.g., linoleic and linolenic acids) derived from host are the major target of ROS in the Lyme disease spirochete.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk