Assembly of multilayer PSS/PAH membrane on coherent alginate/PLO microcapsule for long-term graft transplantation

J Biomed Mater Res A. 2009 Jan;88(1):226-37. doi: 10.1002/jbm.a.31891.

Abstract

Conventional alginate/poly-L-ornithine (AP) membranes used to immunoisolate foreign tissue transplants fail in long-term transplantations of immortal cell lines. We have developed a novel layer-by-layer (LbL) membrane using polystyrene sulfonate and polyallylamine hydrochloride (PSS/PAH) on top of the coherent AP membrane. Assembly of the LbL membrane was followed by electrophoresis, and the surface morphologies and structure were characterized and examined by cryo-scanning electron microscope and transmission electron microscopy. Unlike the standard AP membrane, the LbL membrane withstood the internal pressure generated by continuous cell proliferation of microencapsulated HEK-293 and Min-6 cells. The new membrane did not affect insulin secretion or diffusion by Min-6 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsules / chemistry*
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Transplantation / methods*
  • Humans
  • Insulin / metabolism
  • Insulin Secretion
  • Kidney / cytology*
  • Membranes, Artificial*
  • Polyamines
  • Polystyrenes
  • Surface Properties

Substances

  • Capsules
  • Insulin
  • Membranes, Artificial
  • Polyamines
  • Polystyrenes
  • polyallylamine
  • polystyrene sulfonic acid