Curr Opin Chem Biol. 2008 Feb;12(1):55-9. Epub 2008 Mar 7.

New insights into chemical biology from ORFeome libraries.

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Chemical Genetics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

Abstract

As the genomes of many organisms have been sequenced, a variety of global analyses, called 'omics,' have been initiated. Cloning of the set of all open reading frames encoded by the genome (ORFeome) of an organism is a major challenge, which serves as an indispensable provision before one launches into the ocean of the postgenomic world. A suitable strategy for high-throughput cloning and expression of thousands of genes is crucial to success. Recently developed systems employing site-specific or homologous recombination have made it feasible to manipulate thousands of ORFs en masse. Using these technologies, several recent studies have successfully fished biologically active small molecules and target proteins out of this bountiful ocean.

PMID:: 18282487; [PubMed - indexed for MEDLINE]

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Systematic cloning of an ORFeome using the Gateway system. [Methods Mol Biol. 2009]
Systematic cloning of an ORFeome using the Gateway system.
Matsuyama A, Yoshida M. Methods Mol Biol. 2009; 577:11-24.
Review FLEXGene repository: from sequenced genomes to gene repositories for high-throughput functional biology and proteomics. [Mol Biochem Parasitol. 2001]
Review FLEXGene repository: from sequenced genomes to gene repositories for high-throughput functional biology and proteomics.
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Review ORFeome projects: gateway between genomics and omics. [Curr Opin Chem Biol. 2004]
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Matsuyama A, Arai R, Yashiroda Y, Shirai A, Kamata A, Sekido S, Kobayashi Y, Hashimoto A, Hamamoto M, Hiraoka Y, et al. Nat Biotechnol. 2006 Jul; 24(7):841-7. Epub 2006 Jun 25.

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