Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster

Fish Shellfish Immunol. 2008 Mar;24(3):366-71. doi: 10.1016/j.fsi.2007.12.001. Epub 2007 Dec 8.

Abstract

By using EDTA and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, Conger myriaster. We then identified TNF decoy receptor (DcR) cDNA in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with LPS. The full-length cDNA of conger TNF DcR (conDcR) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. Phylogenetic analysis indicated that conDcR was clustered into a DcR3 branch. ConDcR is likely to act as an important immune-regulating factor in inhibiting the apoptosis-inducing effect of TNF in the skin of conger eel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary
  • Eels / genetics*
  • Epidermal Cells*
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Tumor Necrosis Factor Decoy Receptors / chemistry
  • Tumor Necrosis Factor Decoy Receptors / genetics*

Substances

  • DNA, Complementary
  • Tumor Necrosis Factor Decoy Receptors

Associated data

  • GENBANK/AB295668