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Rev Sci Instrum. 2008 Jan;79(1):013708. doi: 10.1063/1.2830286.

A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays.

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  • 1Department of Physics, University of California at Davis, Davis, California 95616, USA.


We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.

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