(A) The quaternary complex of IL-2 (pink), IL-2Rα (cyan), IL-2Rβ (blue) and γ_c (gold). IL-2Rα is bound to the top surface of IL-2. (B) Ternary complex of IL-13 (yellow-orange), IL-4Rα (blue), and IL-13Rα1 (green). (C) As viewed from the top, the overlapping cytokine binding sites by IL-2Rα and IL-13Rα1 D1 domains are apparent. Semi-transparent ovals have been drawn around the D1 domain of IL-2Rα and IL-13Rα1 so that regions of overlap are clear.

For clarity the overall complexes are shown above blowups of the site I IL-4 (A) and IL-13 (B) interfaces, with color scheme retained from Figure 1. Zoomed up region of the site I interfaces focuses on the IL-4Rα EF loop interactions with cytokine A and C helices (labeled). Key interacting residues are shown in stick representation, and the “mimicked” Arg and Glu hotspot residues found in both IL-4 and IL-13 are thicker sticks and colored cyan, with polar contacts drawn as dashed green lines. (C) “Open book” view of binding epitopes presented by IL-4 and IL-13 to IL-4Rα. The resulting IL-4 and IL-13 cytokine (shown as cylinders) positions after their respective IL-4Rα were superimposed shows the relative structural overlap of the cytokine site I binding sites. (D) Structure-based sequence alignment between the interacting portions of helices A and C of IL-4 and IL-13 with IL-4Rα, where contact positions are in red boxes, common hydrophobic core residues are highlighted in gray and “hot spot” residues are highlighted in cyan to coincide with panels (A), (B) and (C).

(A) Structural alignment of the IL-2 quaternary (IL-2Rα not shown), and IL-4 Type I ternary complexes after superposition on γ_c (gold). The IL-2 complex is blue, and the IL-4 complex is green. (B) The conserved apolar ‘canyon’ on the cytokine surfaces (highlighted in blue) accommodates the protruding γ_c binding loops (gold and silver residues). (C) “Open book” views of the overlap of IL-2 versus IL-4 site IIa binding sites after superposition of γ_c. (D) Structure-based sequence alignment of all known γ_c cytokines for helices A and D based on γ_c contact positions by IL-2 and IL-4. Residue numbers are included and contact residues from IL-2 and IL-4 are marked with red boxes. Hydrophobic residues are shaded in gray and correspond predominantly with buried core residues.

(A) The Type I complex between IL-4Rα (blue), IL-4 (red) and γ_c (gold). (B) The Type II IL-4 complex between IL-4Rα (blue), IL-4 (red) and IL-13Rα1 (green). (C) The Type II IL-13 complex with IL-4Rα (blue), IL-13 (yellow-orange) and IL-13Rα1 (green). The complexes are shown from the side with a cartoon of a membrane underneath (left), and as viewed from the “top” (right). Glycan moieties on Asn residues are shown in ball-and-stick representation. All figures were generated with PyMol (DeLano, The PyMOL Molecular Graphics System, 2002).

Each row illustrates the reaction in the order in which the binary and ternary complexes assemble (color scheme is maintained from Figure 1). Inset tables list the thermodynamic parameters calculated based upon the isothermal titration calorimetry (ITC) binding isotherm shown below each reaction diagram. Data are also summarized in Table S3 (A) Type I complex: Reaction diagram for the interaction of IL-4Rα and IL-4 followed by recruitment of the second receptor, γ_c. (B) Type II IL-4 complex: the interaction of IL-4Rα and IL-4 followed by the recruitment of the second receptor, IL-13Rα1. In both (A) and (B), the extremely high affinity of IL-4/IL-4Rα (K_d < 1nM) is an estimate due to the steepness of the ITC trace. (C) Type II IL-13 complex: the interaction of IL-13Rα1 and IL-13 followed by the recruitment of the second receptor, IL-4Rα.

For clarity the overall complexes are shown in between blowups of the site IIa (underneath) and III (above) interfaces using the same color scheme as previous figures. Panels (A), (B) and (C) show zoomed views of the site IIa interfaces of the IL-4 Type I, IL-4 Type II, and IL-13 Type II complexes, respectively, where the receptor residues are projected on semi-transparent cytokine surfaces. IL-4(red) and IL-13(yellow-orange) residues underneath the surfaces are labeled in white and black, respectively, while γ_c (gold) and IL-13 Rα1(green) residues are labeled in yellow and green, respectively. (D) and (E) “Open book” views of the site IIa binding sites. Panel (D) shows the resulting structural positions of IL-4 on IL-4 after superposition of IL-4Rα from the Type I and Type II complexes, and panel (E) shows the resulting structural positions of IL-4 on IL-13 after superposition of IL-4Rα from the two different Type II complexes. All interacting residues in the site IIa interfaces are shown as sticks. Panels (F) and (G) illustrate the site III interfaces in IL-4 and IL-13 Type II complexes, respectively, using a similar coloring and labeling scheme as above. The anti-parallel beta sheet between the IL-13Rα1 c’ strand the cytokine C–D loop is labeled.

(A) A549 express IL-4Rα and IL-13Rα1, but not IL-13Rα2 or γ_c. Expression of receptor subunits on A549 was analyzed by FACS as described in Methods. The heavy black histograms indicate staining with specific antibody and the dotted histograms indicate staining by the isotype-matched control. (B) A549 cells and Ramos cells were washed and treated with various concentrations of IL-4 or IL-13 as indicated for 30 minutes. Cell lysates were prepared and immunoprecipitated with anti-STAT6. Western blots were probed with anti-phosphotyrosine antibody. The blots were stripped and reprobed with anti-STAT6. STAT3 activation is analyzed in Figure S6. (C) A549 cells and Ramos cells were treated with various concentrations of IL-4 or IL-13 for various times; STAT6 phosphorylation was analyzed as described in (B). Data is quantitated in graphical format in Figure S7.