Format

Send to

Choose Destination
See comment in PubMed Commons below
Science. 2008 Feb 1;319(5863):594-6. doi: 10.1126/science.1151424.

Single-molecule cut-and-paste surface assembly.

Author information

  • 1Center for Nanoscience and Department of Physics, University of Munich, Amalienstrasse 54, 80799 Munich, Germany.

Abstract

We introduce a method for the bottom-up assembly of biomolecular structures that combines the precision of the atomic force microscope (AFM) with the selectivity of DNA hybridization. Functional units coupled to DNA oligomers were picked up from a depot area by means of a complementary DNA strand bound to an AFM tip. These units were transferred to and deposited on a target area to create basic geometrical structures, assembled from units with different functions. Each of these cut-and-paste events was characterized by single-molecule force spectroscopy and single-molecule fluorescence microscopy. Transport and deposition of more than 5000 units were achieved, with less than 10% loss in transfer efficiency.

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk