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Eur J Pharmacol. 2008 Mar 10;581(3):235-43. doi: 10.1016/j.ejphar.2007.11.060. Epub 2007 Dec 14.

Mu-opioid receptor heterooligomer formation with the dopamine D1 receptor as directly visualized in living cells.

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  • 1Department of Pharmacology University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada M5S 1A8.

Abstract

Our immunohistochemistry experiments demonstrated that the mu-opioid receptor co-localized with the dopamine D1 receptor in neurons of the cortex and caudate nucleus. On the basis of this physiological data we further investigated whether these two G protein coupled receptors formed hetero-oligomers in living cells. To demonstrate hetero-oligomerization we used a novel strategy, the method used harnessed the physiological cellular mechanism for transport of proteins to the nucleus. The nuclear translocation pathway was adapted for the visualization of mu-opioid hetero-oligomers with the dopamine D1 receptor. The receptor hetero-oligomer complex formed resulted in a significantly enhanced surface expression of mu-opioid receptor. This hetero-oligomer formation involved the interaction of mu-opioid receptor with the dopamine D1 receptor carboxyl tail, since a dopamine D1 receptor substituted with the carboxyl of the dopamine D5 receptor failed to increase surface expression of mu-opioid receptor.

PMID:
18237729
[PubMed - indexed for MEDLINE]
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