Structural studies on flavohemoglobins

Methods Enzymol. 2008:436:187-202. doi: 10.1016/S0076-6879(08)36010-8.

Abstract

The key three-dimensional features of flavohemoglobins have been unveiled by X-ray crystallographic investigations carried out on the Alcaligenes eutrophus and Escherichia coli proteins. Flavohemoglobins are made of a globin domain fused with a ferredoxin reductase-like FAD binding module and display highly conserved sequences in the active sites of both the heme-binding domain and the flavin-binding domain. Structural studies are discussed and methodological approaches to the solution of the crystal structures and to the analysis of the relevant stereochemical properties of the active sites are presented. The understanding of the structural properties of flavohemoglobins serves as a guide for testing biological hypotheses and allows for a rational evaluation of structure-based alignments within the flavohemoglobin family.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Crystallography, X-Ray
  • Cupriavidus necator / chemistry
  • Cupriavidus necator / genetics
  • Dihydropteridine Reductase / chemistry*
  • Dihydropteridine Reductase / genetics
  • Dihydropteridine Reductase / metabolism
  • Escherichia coli / chemistry
  • Escherichia coli / genetics
  • Escherichia coli Proteins / chemistry*
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Flavin-Adenine Dinucleotide / metabolism
  • Heme / metabolism
  • Hemeproteins / chemistry*
  • Hemeproteins / genetics
  • Hemeproteins / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • NAD / metabolism
  • NADH, NADPH Oxidoreductases / chemistry*
  • NADH, NADPH Oxidoreductases / genetics
  • NADH, NADPH Oxidoreductases / metabolism
  • Phospholipids / chemistry
  • Protein Folding
  • Protein Structure, Tertiary
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • Hemeproteins
  • Phospholipids
  • flavohemoprotein, Bacteria
  • NAD
  • Flavin-Adenine Dinucleotide
  • Heme
  • Dihydropteridine Reductase
  • hmp protein, E coli
  • NADH, NADPH Oxidoreductases