Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2008 Feb;5(2):135-46. doi: 10.1038/nmeth.f.202.

Protein production and purification.

Author information

  • 1Karolinska Institutet, Schéeles väg 2, 171 77 Stockholm, Sweden.

Erratum in

  • Nat Methods. 2008 Apr;5(4):369. Hallberg, B Martin [added].


In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Write to the Help Desk