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Nat Methods. 2008 Feb;5(2):135-46. doi: 10.1038/nmeth.f.202.

Protein production and purification.

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  • 1Karolinska Institutet, Schéeles väg 2, 171 77 Stockholm, Sweden.

Erratum in

  • Nat Methods. 2008 Apr;5(4):369. Hallberg, B Martin [added].

Abstract

In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.

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